Re: New planted tank... fish are dying.
First, it may be that the pleco is just such a sedentary fish that you can't tell if he is stressed.
Second, one pleco in a newly planted tank is just starting a fishless cycle. One fish can only produce so much waste, so the balance between plants and fish developed slowly. It takes a while for the fish to pollute the big tank full of fresh water, meanwhile, the plants are stripping out ammonia as fast as the lighting will allow them to run. You said the plants were growing like crazy, so they were taking in a lot of ammonia.
I'm not sure how or when you used rainwater, and it may be that one night a plane passed over and sprayed and the toxin landed on your roof. Depending on when it rained and when you collected the water, you may or may not have gotten any toxins, even if the plane did pass over your house. Another collection may be just fine. I dunno. But, you can ponder that one. I still think your issues are just cycling.
So, all was well in the tank, plants sucking up all the ammonia they could handle, then you added some sensitive fish. And fed them. So, now there is a burst of ammonia, more than the plants could handle. It may also be that the plants have begun to exhaust some of the nutritents they had stored in plant tissues. If the plants were running low on vital nutrients, at the same time ammonia increased with more fish.... you may get some ammonia, then some nitrites in the water, eventually nitrates too. So the fish look stressed.
Now, if you dump in rainwater, the change of water hardness alone can be a big thing. Conditioning fish can be critical when changing water - from the store to tap to rainwater may be too many changes. I'm not sure about the sequence of events here. I'm just listing the probably causes.
Some stores use reconstituted RO water in their tanks (Aquarium world) others use tap water. Some may use chemicals. I dunno. Always good to ask when making a purchase, unless you are pretty sure it is tap water.
Next, tend to the plants. I do hate to tell you to buy yet another thing, but Tropic Master Grow (Village Tropical or online) is a good place to start with fertilizing plants. Otherwise, you may try root fertilizers, I forget what the brand names are but do not use yard stuff, use fish tank stuff. Finally, you can order what you need from online sources, try Greg Watson.com. I do not like the typical plant fertilizers from the store, I've always gotten alage, not sure what they are too high in, but they seem unbalanced.
As for the fish, yes, I think the pleco should go back now. With only two rams left, that is not much of a load on the planted tank. Before you move them, test for water hardness. Do a water change on the planted tank so that both tanks have similar water composition, tap or rainwater, whatever. If you are worried about the rainwater, then slowly go to tap in both tanks. Now or later, when the tank waters are the same, move all fish and all filters to the new tank. Let those filters run together for a month if you can.
First, it may be that the pleco is just such a sedentary fish that you can't tell if he is stressed.
Second, one pleco in a newly planted tank is just starting a fishless cycle. One fish can only produce so much waste, so the balance between plants and fish developed slowly. It takes a while for the fish to pollute the big tank full of fresh water, meanwhile, the plants are stripping out ammonia as fast as the lighting will allow them to run. You said the plants were growing like crazy, so they were taking in a lot of ammonia.
I'm not sure how or when you used rainwater, and it may be that one night a plane passed over and sprayed and the toxin landed on your roof. Depending on when it rained and when you collected the water, you may or may not have gotten any toxins, even if the plane did pass over your house. Another collection may be just fine. I dunno. But, you can ponder that one. I still think your issues are just cycling.
So, all was well in the tank, plants sucking up all the ammonia they could handle, then you added some sensitive fish. And fed them. So, now there is a burst of ammonia, more than the plants could handle. It may also be that the plants have begun to exhaust some of the nutritents they had stored in plant tissues. If the plants were running low on vital nutrients, at the same time ammonia increased with more fish.... you may get some ammonia, then some nitrites in the water, eventually nitrates too. So the fish look stressed.
Now, if you dump in rainwater, the change of water hardness alone can be a big thing. Conditioning fish can be critical when changing water - from the store to tap to rainwater may be too many changes. I'm not sure about the sequence of events here. I'm just listing the probably causes.
Some stores use reconstituted RO water in their tanks (Aquarium world) others use tap water. Some may use chemicals. I dunno. Always good to ask when making a purchase, unless you are pretty sure it is tap water.
Next, tend to the plants. I do hate to tell you to buy yet another thing, but Tropic Master Grow (Village Tropical or online) is a good place to start with fertilizing plants. Otherwise, you may try root fertilizers, I forget what the brand names are but do not use yard stuff, use fish tank stuff. Finally, you can order what you need from online sources, try Greg Watson.com. I do not like the typical plant fertilizers from the store, I've always gotten alage, not sure what they are too high in, but they seem unbalanced.
As for the fish, yes, I think the pleco should go back now. With only two rams left, that is not much of a load on the planted tank. Before you move them, test for water hardness. Do a water change on the planted tank so that both tanks have similar water composition, tap or rainwater, whatever. If you are worried about the rainwater, then slowly go to tap in both tanks. Now or later, when the tank waters are the same, move all fish and all filters to the new tank. Let those filters run together for a month if you can.

Every fish I have goes crazy for bloodworms. If they're smaller, try getting the frozen Hikari bloodworms, those worms are smaller than most other brands and can be eaten by the little guys.
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